A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots

doi:10.1039/c6ra13325h

NWIPB OpenIR

	A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots
	Zhao, X. E.1,2; Lei, C. H.1; Wang, Y. H.1; Qu, F.1; Zhu, S. Y.1; Wang, H.1; You, J. M.1,2
	2016
发表期刊	RSC ADVANCES
卷号	6 期号:76 页码:72670-72675
文章类型	Article
摘要	In this work, a sensitive and selective fluorescence sensing platform was developed for the detection of tyrosinase (TYR) activity and its inhibitor screening using graphene quantum dots (GQDs) as probes. Upon excitation at 383 nm, GQDs displayed an intense emission at 445 nm. TYR, a typical polyphenol oxidase, can catalyze the oxidation of dopamine (DA) to dopaquinone and then a fluorescence resonance energy transfer (FRET) process between GQDs and dopaquinone took place. Therefore, the fluorescence of GQDs could be quenched. Thus, quantitative evaluation of TYR activity was established in terms of the relationship between fluorescence quenching efficiency and TYR activity. A linear range was obtained from 0.005 to 0.5 U mL(-1) and from 0.5 to 5.0 U mL(-1), respectively. A detection limit of 0.0015 U mL(-1) was obtained, which was lower than those of reported papers. In addition, it constructed a useful platform for TYR inhibitor screening.
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1039/c6ra13325h
关键词[WOS]	PROBING BIOCATALYTIC TRANSFORMATIONS ; FLUORESCENT-PROBE ; LABEL-FREE ; ULTRASENSITIVE DETECTION ; ACID ; NANOPARTICLES ; NANOCLUSTERS ; ENHANCEMENT ; NANOSENSOR ; DOPAMINE
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2013BQ018) ; Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Student Research Training Program of Qufu Normal University(2015A071) ; Taishan Scholar Foundation of Shandong Province, China ; 81303179 ; 21405093)
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000381513300100
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6449
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zhao, X. E.,Lei, C. H.,Wang, Y. H.,et al. A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots[J]. RSC ADVANCES,2016,6(76):72670-72675.
APA	Zhao, X. E..,Lei, C. H..,Wang, Y. H..,Qu, F..,Zhu, S. Y..,...&You, J. M..(2016).A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots.RSC ADVANCES,6(76),72670-72675.
MLA	Zhao, X. E.,et al."A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots".RSC ADVANCES 6.76(2016):72670-72675.