Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots | |
Zhao, X. E.1,2; Lei, C. H.1; Wang, Y. H.1; Qu, F.1; Zhu, S. Y.1; Wang, H.1; You, J. M.1,2 | |
2016 | |
发表期刊 | RSC ADVANCES |
卷号 | 6期号:76页码:72670-72675 |
文章类型 | Article |
摘要 | In this work, a sensitive and selective fluorescence sensing platform was developed for the detection of tyrosinase (TYR) activity and its inhibitor screening using graphene quantum dots (GQDs) as probes. Upon excitation at 383 nm, GQDs displayed an intense emission at 445 nm. TYR, a typical polyphenol oxidase, can catalyze the oxidation of dopamine (DA) to dopaquinone and then a fluorescence resonance energy transfer (FRET) process between GQDs and dopaquinone took place. Therefore, the fluorescence of GQDs could be quenched. Thus, quantitative evaluation of TYR activity was established in terms of the relationship between fluorescence quenching efficiency and TYR activity. A linear range was obtained from 0.005 to 0.5 U mL(-1) and from 0.5 to 5.0 U mL(-1), respectively. A detection limit of 0.0015 U mL(-1) was obtained, which was lower than those of reported papers. In addition, it constructed a useful platform for TYR inhibitor screening. |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1039/c6ra13325h |
关键词[WOS] | PROBING BIOCATALYTIC TRANSFORMATIONS ; FLUORESCENT-PROBE ; LABEL-FREE ; ULTRASENSITIVE DETECTION ; ACID ; NANOPARTICLES ; NANOCLUSTERS ; ENHANCEMENT ; NANOSENSOR ; DOPAMINE |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2013BQ018) ; Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Student Research Training Program of Qufu Normal University(2015A071) ; Taishan Scholar Foundation of Shandong Province, China ; 81303179 ; 21405093) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000381513300100 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6449 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, X. E.,Lei, C. H.,Wang, Y. H.,et al. A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots[J]. RSC ADVANCES,2016,6(76):72670-72675. |
APA | Zhao, X. E..,Lei, C. H..,Wang, Y. H..,Qu, F..,Zhu, S. Y..,...&You, J. M..(2016).A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots.RSC ADVANCES,6(76),72670-72675. |
MLA | Zhao, X. E.,et al."A fluorometric assay for tyrosinase activity and its inhibitor screening based on graphene quantum dots".RSC ADVANCES 6.76(2016):72670-72675. |
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