Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography | |
Dang, Jun; Ma, Jianbin; Du, Yurong; Dawa, YangZom; Wang, Qi; Chen, Chengbiao; Wang, Qilan; Tao, Yanduo; Ji, Tengfei | |
2021 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
卷号 | 1170 |
摘要 | In this study, polyamide and MCI GEL? CHP20P were employed as stationary phases in medium pressure chromatography (MPC) for the efficient preparative separation of bergenin from Saxifraga atrata. Ethanol?water, methanol-water, and acetonitrile-water mobile phases all showed good enrichment capacity for bergenin fraction when polyamide was used as a stationary phase. After 5 cycles of polyamide MPC using acetonitrile/water, 1.2 g of bergenin fraction was isolated from 180 g Saxifraga atrata herb. Further purification of this fraction was conducted using MCI GEL? CHP20P styrene-divinylbenzene beads. The bergenin fraction was separated into two fractions, and after three runs of MPC, 714.2 mg of bergenin with purity above 99% was obtained. The results demonstrate that the combination of polyamide and styrene-divinylbenzene MPC can be utilized for preparative isolation of compounds from natural products with high yield and purity. |
关键词 | Saxifraga atrata Polyamide Micro gel chp20p Medium pressure chromatography Bergenin |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60815 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Dang, Jun,Ma, Jianbin,Du, Yurong,et al. Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2021,1170. |
APA | Dang, Jun.,Ma, Jianbin.,Du, Yurong.,Dawa, YangZom.,Wang, Qi.,...&Ji, Tengfei.(2021).Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1170. |
MLA | Dang, Jun,et al."Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1170(2021). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论