Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection

doi:10.1007/s10337-016-3174-1

NWIPB OpenIR

	Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection
	Wang, Yuwei 1,3; Suo, Yourui 1,2; Sun, Yanan 1,3; You, Jinmao 1,2
	2016-11-01
发表期刊	CHROMATOGRAPHIA
卷号	79 期号:21-22 页码:1515-1525
文章类型	Article
摘要	In this work, we have developed an efficient method for the rapid extraction and separation of triterpene acids from 37 different varieties of raspberry via ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME). The triterpene acids were then determined by high-performance liquid chromatography (HPLC) with fluorescence detection using benzimidazo-[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate (BQETS) as the labeling agent. Five triterpene acids, including asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and betulinic acid (BA), were extracted by UA-DLLME using chloroform and acetone as the extracting and dispersing solvents, respectively. After extraction and nitrogen flushing, the extracts were simultaneously characterized by HPLC based on pre-column derivatization using BQETS, a new labeling agent synthesized in our laboratory. Several key parameters affecting the extraction efficiency and derivatization yields were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The method was further validated for linearity (correlation coefficient R (2) > 0.9979), precision (RSD = 0.23-2.45 %), and recovery (RSD = 90-106.5 %). The limits of detection (LODs) and the limits of quantification (LOQs) were determined to be within the range of 1.83-7.69 A mu g/L and 6.06-25.47 A mu g/L, respectively. This is the first report of the use of BQETS as a pre-column derivatization agent for the determination of triterpene acids in real samples. The proposed method has been applied to the determination of five triterpene acids in 37 different raspberry varieties with significantly increased sensitivity compared to other methods. The results obtained indicate that the contents of triterpene acids vary significantly across different raspberry varieties.
关键词	Ultrasound-assisted Dispersive Liquid-liquid Microextraction (Ua-dllme) Pre-column Derivatization High-performance Liquid Chromatography (Hplc) Raspberry Benzimidazo-[2 Triterpene Acids 1-b] Quinazolin-12(6h)-one-5-ethyl-p-toluenesulfonate (Bqets)
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1007/s10337-016-3174-1
关键词[WOS]	LIQUID-LIQUID MICROEXTRACTION ; THIN-LAYER-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; OLEANOLIC ACID ; URSOLIC ACID ; MS METHOD ; ANTIOXIDANT CAPACITY ; RED RASPBERRIES ; QUANTIFICATION ; ELLAGITANNINS
收录类别	SCI
语种	英语
项目资助者	major science and technology projects of Qinghai Province: utilization and development with high value and further process technology of medlar(2015-NK-A2) ; unit of science and technology office of Qinghai province: manufacture integration technology and demonstration of high standards medlar with antioxidant and anticohesion(2015-SF-120)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000387326700009
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6670
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuwei,Suo, Yourui,Sun, Yanan,et al. Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection[J]. CHROMATOGRAPHIA,2016,79(21-22):1515-1525.
APA	Wang, Yuwei,Suo, Yourui,Sun, Yanan,&You, Jinmao.(2016).Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection.CHROMATOGRAPHIA,79(21-22),1515-1525.
MLA	Wang, Yuwei,et al."Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection".CHROMATOGRAPHIA 79.21-22(2016):1515-1525.