Isolation and characterization of a subgroup IIa WRKY transcription factor PtrWRKY40 from Populus trichocarpa

doi:10.1093/treephys/tpv084

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	Isolation and characterization of a subgroup IIa WRKY transcription factor PtrWRKY40 from Populus trichocarpa
	Karim, Abdul 1; Jiang, Yuanzhong 1; Guo, Li 1; Ling, Zhengyi 1; Ye, Shenglong 1; Duan, Yanjiao 1; Li, Chaofeng 2; Luo, Keming 1,2
	2015-10-01
发表期刊	TREE PHYSIOLOGY
卷号	35 期号:10 页码:1129-1139
文章类型	Article
摘要	Salicylic acid (SA) is a defense-related key signaling molecule involved in plant immunity. In this study, a subgroup IIa WRKY gene PtrWRKY40 was isolated from Populus trichocarpa, which displayed amino acid sequence similar to Arabidopsis AtWRKY40, AtWRKY18 and AtWRKY60. PtrWRKY40 transcripts accumulated significantly in response to SA, methyl jasmonate and hemibiotrophic fungus Dothiorella gregaria Sacc. Overexpression of PtrWRKY40 in transgenic poplar conferred higher susceptibility to D. gregaria infection. This susceptibility was coupled with reduced expression of SA-associated genes (PR1.1, PR2.1, PR5.9, CPR5 and SID2) and jasmonic acid (JA)-related gene JAZ8. Decreased accumulation of endogenous SA was observed in transgenic lines overexpressing PtrWRKY40 when compared with wild-type plants. However, constitutive expression of PtrWRKY40 in Arabidopsis thaliana displayed resistance to necrotrophic fungus Botrytis cinerea, and the expression of JA-defense-related genes such as PDF1.2, VSP2 and PR3 was remarkably increased in transgenic plants upon infection with fugal pathogens. Together, our findings indicate that PtrWRKY40 plays a negative role in resistance to hemibiotrophic fungi in poplar but functions as a positive regulator of resistance toward the necrotrophic fungi in Arabidopsis.
关键词	Jasmonate Pathogen Resistance Populus Salicylic Acid
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1093/treephys/tpv084
关键词[WOS]	PLANT DEFENSIN GENE ; SALICYLIC-ACID ; ARABIDOPSIS-THALIANA ; SIGNALING PATHWAYS ; RESPONSE PATHWAYS ; MISSING LINK ; DNA-BINDING ; CROSS-TALK ; RESISTANCE ; JASMONATE
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31370672 ; National Key Project for Research on Transgenic Plant(2011ZX08010-003) ; Fundamental Research Funds for the Central Universities(XDJK2014a005) ; Natural Science Foundation Project of CQ CSTC(CSTC2013JJB8007) ; 'One Hundred Talents Program' of the Chinese Academy of Sciences ; 31171620)
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000366522800010
引用统计	被引频次：44[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6518
专题	中国科学院西北高原生物研究所
作者单位	1.Southwest Univ, Key Lab Ecoeenvironm Three Gorges Reservoir Reg, Chongqing Key Lab Transgen Plant & Safety Control, Sch Life Sci,Minist Educ,Inst Resources Bot, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Karim, Abdul,Jiang, Yuanzhong,Guo, Li,et al. Isolation and characterization of a subgroup IIa WRKY transcription factor PtrWRKY40 from Populus trichocarpa[J]. TREE PHYSIOLOGY,2015,35(10):1129-1139.
APA	Karim, Abdul.,Jiang, Yuanzhong.,Guo, Li.,Ling, Zhengyi.,Ye, Shenglong.,...&Luo, Keming.(2015).Isolation and characterization of a subgroup IIa WRKY transcription factor PtrWRKY40 from Populus trichocarpa.TREE PHYSIOLOGY,35(10),1129-1139.
MLA	Karim, Abdul,et al."Isolation and characterization of a subgroup IIa WRKY transcription factor PtrWRKY40 from Populus trichocarpa".TREE PHYSIOLOGY 35.10(2015):1129-1139.

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