Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design | |
Yang, Jingchang1,2; Rong, Huan1,2; Shao, Ping3; Tao, Yanduo3; Dang, Jun3; Wang, Peng4; Ge, Yushu1,2; Wu, Jiang4; Liu, Dan1,2 | |
2016 | |
发表期刊 | JOURNAL OF MATERIALS CHEMISTRY B |
卷号 | 4期号:36页码:6065-6073 |
文章类型 | Article |
摘要 | Recently, fluorescent peptide-based chemosensors have been reported and used to detect series of metal ions in both aqueous solutions and living cells. In order to explore and indicate the structural regulations of ''fluorophore-(His)(n)-Pro-Gly-(His)(n)-fluorophore (H2L peptide sensor)'', we predict the relationship between the number of histidine molecules and the coordination activity, as well as the preferred coordination site, through computational studies. L-n=3 was modeled as showing the highest activity with the lowest calculated DEtotal, and inner histidine residues bonding with the ''Pro-Gly'' core structure always provided essential coordination sites for zinc ions. A predicted structural regulation mechanism was studied and verified experimentally. Three Zn2+ sensors with the number of histidine molecules set as 2 (L-n=2), 3 (L-n=3) and 4 (L-n=4) were synthesized and tested. The L-n=3 sensor shows the highest fluorescence intensity by specific binding with Zn2+. The key coordination site was identified by mutating the histidine residues of the L-n=3 sensor. The inner histidine bonding with the ''Pro-Gly'' core structure was proved as the vital coordination site, and at least one extra histidine had to be provided to assist the coordination process according to the experimental results. Both computational and experimental studies reveal the same relationship between the structure and activity for H2L peptides in the fluorescent sensing of zinc ions. The results give fundamental information for the reasonable design and optimization of all similar classes of peptide sensors. |
WOS标题词 | Science & Technology ; Technology |
DOI | 10.1039/c6tb01570k |
关键词[WOS] | FLUORESCENT CHEMOSENSOR ; METAL-IONS ; AQUEOUS-SOLUTIONS ; HG(II) IONS ; AMINO-ACID ; SENSORS ; COPPER ; CU |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Recruitment Program for Young Professionals(KJ2070000027) ; Major State Special Research Project of China(2016YFA0101200) ; Major State Basic Research Development Program of China (973 Program)(2015CB553701) ; National Natural Science Foundation of China(21201091) ; Fundamental Research Funds for the Central Universities(lzujbky-2013-59) |
WOS研究方向 | Materials Science |
WOS类目 | Materials Science, Biomaterials |
WOS记录号 | WOS:000384233900011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6436 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Univ Sci & Technol China, Inst Immunol, Collaborat Innovat Ctr Chem Life Sci, Hefei 230027, Peoples R China 2.Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, Sch Life Sci, Hefei 230027, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 4.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Jingchang,Rong, Huan,Shao, Ping,et al. Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design[J]. JOURNAL OF MATERIALS CHEMISTRY B,2016,4(36):6065-6073. |
APA | Yang, Jingchang.,Rong, Huan.,Shao, Ping.,Tao, Yanduo.,Dang, Jun.,...&Liu, Dan.(2016).Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design.JOURNAL OF MATERIALS CHEMISTRY B,4(36),6065-6073. |
MLA | Yang, Jingchang,et al."Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design".JOURNAL OF MATERIALS CHEMISTRY B 4.36(2016):6065-6073. |
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