LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling

NWIPB OpenIR

	LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling
	Liu, WJ; Jiang, XY; Li, X; Sun, KX; Yang, YM; Yang, M; Li, SJ; Zhu, XJ
	2022
发表期刊	JOURNAL OF CELL SCIENCE
卷号	135 期号:6
摘要	Precise Norrin and beta-catenin (Norrin/beta-catenin; encoded by NDP and CTNNB1, respectively) signaling is critical for proper angiogenesis. Dysregulation of this signaling leads to various diseases, of which retinal exudative vitreoretinopathy is the most prevalent. Here, we used a global knockout mouse model to show that limb development membrane protein 1 like (LMBR1L), a transmembrane protein of unknown function in angiogenesis, is essential for retinal vascular development. In vitro experiments revealed that LMBR1L depletion results in aberrant activation of the Norrin/beta-catenin signaling pathway via decreased ubiquitylation of FZD4 and increased Norrin coreceptor LRP5 and p-GSK38-Ser9 expression levels, which cause accumulation of beta-catenin. Moreover, inhibition of LMBR1L in human retinal microvascular endothelial cells (HRECs) caused increased proliferation ability and defective cell migration, which might have occurred as a result of upregulated expression levels of the apical junction components. Treatment with p-GSK3 beta-Ser9 inhibitor AR-A014418 restored the phenotypes in LMBR1L null HRECs, which further demonstrated the important regulatory role of LMBR1L in the Norrin/beta-catenin signaling pathway. Taken together, our data reveal an essential role for LMBR1L in angiogenesis.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61277
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, WJ,Jiang, XY,Li, X,et al. LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling[J]. JOURNAL OF CELL SCIENCE,2022,135(6).
APA	Liu, WJ.,Jiang, XY.,Li, X.,Sun, KX.,Yang, YM.,...&Zhu, XJ.(2022).LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling.JOURNAL OF CELL SCIENCE,135(6).
MLA	Liu, WJ,et al."LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling".JOURNAL OF CELL SCIENCE 135.6(2022).