Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata and their antioxidant activities

NWIPB OpenIR

	Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata and their antioxidant activities
	Fang, Y; Dawa, Y; Wang, QL; Lv, Y; Yu, W; Li, G; Dang, J
	2022
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	45 期号:14 页码:2435
摘要	Saxifraga atrata is an important traditional Tibetan medicine used to treat cough and pneumonia, and has tremendous medicinal potential. In this study, we devised a technique to separate 1,1-diphenyl-2-picrylhydrazyl inhibitors from a methanol extract of S. atrata. The material was first processed using MCI GEL CHP20P medium-pressure liquid chromatography, yielding 1.1 g of the target fraction Fr2. Subsequently, online hydrophilic interaction liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay was used to identify prospective 1,1-diphenyl-2-picrylhydrazyl inhibitors, and two 1,1-diphenyl-2-picrylhydrazyl inhibitor fractions (Fr24 and Fr25) were identified from Fr2. Then, medium-pressure preparation was continued using an XIon column to separate two 1,1-diphenyl-2-picrylhydrazyl inhibitor fractions (Fr24 and Fr25). The target compound was concentrated in fractions Fr24 and Fr25 using reverse-phase liquid chromatography during further separation procedures. Finally, the purity, structure, and 1,1-diphenyl-2-picrylhydrazyl inhibitory activity of the isolated 1,1-diphenyl-2-picrylhydrazyl inhibitors were determined. Two 1,1-diphenyl-2-picrylhydrazyl inhibitors (adenosine with the half maximal inhibitory concentration of 66.87 +/- 14.33 mu M and (-)-4-O-(E)-caffeoyl-l-threonic acid with the half maximal inhibitory concentration of 59.06 +/- 5.02 mu M) were isolated with purities exceeding 95%. The results showed that this technology is effective in the targeted separation of antioxidants from natural products.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61216
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fang, Y,Dawa, Y,Wang, QL,et al. Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata and their antioxidant activities[J]. JOURNAL OF SEPARATION SCIENCE,2022,45(14):2435.
APA	Fang, Y.,Dawa, Y.,Wang, QL.,Lv, Y.,Yu, W.,...&Dang, J.(2022).Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata and their antioxidant activities.JOURNAL OF SEPARATION SCIENCE,45(14),2435.
MLA	Fang, Y,et al."Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata and their antioxidant activities".JOURNAL OF SEPARATION SCIENCE 45.14(2022):2435.