Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley

NWIPB OpenIR

	Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley
	Jiang, YY; Li, JM; Liu, BL; Cao, D; Zong, Y; Chang, YZ; Li, Y
	2022
发表期刊	TRANSGENIC RESEARCH
摘要	The hordoindolina genes (Hina and Hinb) are believed to play critical roles in barley (Hordeum vulgare L.) grain texture. In this study, we created novel alleles of the Hina gene using CRISPR/Cas9 (Clustered regularly inter spaced short palindromic repeat-associated protein, CRISPR-Cas) genome editing. Mutagenesis of single bases in these novel alleles led to loss of Hina protein function in edited lines. The grain hardness index of hina mutants was 95.5 on average, while that of the wild type was only 53.7, indicating successful conversion of soft barley into hard barley. Observation of cross-sectional grain structure using scanning electron microscopy revealed different adhesion levels between starch granules and protein matrix. Starch granules were loose and separated from the protein matrix in the wild type, but deeply trapped and tightly integrated with the protein matrix in hina02 mutants. In addition, the grain width and thousand-grain weight of the hina02 mutant were significantly lower than those of the wild type.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61132
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jiang, YY,Li, JM,Liu, BL,et al. Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley[J]. TRANSGENIC RESEARCH,2022.
APA	Jiang, YY.,Li, JM.,Liu, BL.,Cao, D.,Zong, Y.,...&Li, Y.(2022).Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley.TRANSGENIC RESEARCH.
MLA	Jiang, YY,et al."Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley".TRANSGENIC RESEARCH (2022).