Prevention properties on cerebral ischemia reperfusion of medicine food homologous Dioscorea yam-derived diosgenin based on mediation of potential targets

NWIPB OpenIR

	Prevention properties on cerebral ischemia reperfusion of medicine food homologous Dioscorea yam-derived diosgenin based on mediation of potential targets
	Zhang, Xinxin; Wang, Xingbin; Xue, Zhaowei; Zhan, Guanqun; Ito, Yoichiro; Guo, Zengjun
	2021
发表期刊	FOOD CHEMISTRY
卷号	345
摘要	I/R (cerebral ischemia reperfusion injury) is the secondary complication of ischemic stroke patients that are immediately treated with drug thrombolysis or vascular recanalization in clinic. Diosgenin (DIO) purified from medicine food homologous (MFH) Dioscorea yam source is served as a fatal starting material to synthesize multifarious steroidal anti-inflammatory drugs in medicinal field, and has previously been demonstrated the potential prevention of I/R. However, the detailed mechanisms of neumprotective effects against I/R remain elusively understood. Here, a global proteomic dynamics of rat right hemisphere brains was executed to investigate the protein expression patterns with a quantitative LC-MSn. In total, 5043 proteins were identified and 418 ones were determined to be significantly dysregulated DEPs (differentially expressed proteins) in comparison of Sham verse I/R and I/R verse DIO after onset stage of I/R, among which 5 DEPs namely BICD2, HNRNPK, CEP41, PPM1K, and ARL2BP, whose biological functions were mainly clustered into the mediation of nervous system, were selected for further validation in vitro and in vivo, and the change tendency expectedly supported the proteomic findings. Additionally, the AUC value of the combined ROC of these 5 DEPs was 0.988 with P < 0.0001, higher than every single one. Collectively, these scientific findings attributed to a typical investigation of dietary Dioscorea-enriched diosgenin in MFH research, suggesting that diosgenin or its derivatives were potential to be developed into food supplements or healthy food products to reveal healthy benefits in natural prevention and reduction risk of I/R. This work also promoted reasonable consumption of Dioscorea yams and contributed to the function of diosgenin-derived products and their applications in food industry.
关键词	Cerebral ischemia reperfusion Diosgenin-derived products Edible Dioscorea yams Medicine food homologous Food therapy
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60800
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Xinxin,Wang, Xingbin,Xue, Zhaowei,et al. Prevention properties on cerebral ischemia reperfusion of medicine food homologous Dioscorea yam-derived diosgenin based on mediation of potential targets[J]. FOOD CHEMISTRY,2021,345.
APA	Zhang, Xinxin,Wang, Xingbin,Xue, Zhaowei,Zhan, Guanqun,Ito, Yoichiro,&Guo, Zengjun.(2021).Prevention properties on cerebral ischemia reperfusion of medicine food homologous Dioscorea yam-derived diosgenin based on mediation of potential targets.FOOD CHEMISTRY,345.
MLA	Zhang, Xinxin,et al."Prevention properties on cerebral ischemia reperfusion of medicine food homologous Dioscorea yam-derived diosgenin based on mediation of potential targets".FOOD CHEMISTRY 345(2021).