Target-Guided Isolation and Purification of Antioxidants from Elsholtzia densa Benth. var. densa by DPPH Antioxidant Assay and Dual-Mode HSCCC

NWIPB OpenIR

	Target-Guided Isolation and Purification of Antioxidants from Elsholtzia densa Benth. var. densa by DPPH Antioxidant Assay and Dual-Mode HSCCC
	Zhou, W. N.; Ouyang, J.; Wang, Z. H.; Wang, X. Y.; Suo, Y. R.; Zhang, Z.; Wang, H. L.
	2018
发表期刊	ACTA CHROMATOGRAPHICA
卷号	30 期号:3
摘要	Elsholtzia densa Benth. var. densa (Lamiaceae) is a famous medicinal herb which has been widely used for treatment of colds, headaches, pharyngitis, fever, diarrhea, digestion disorder, rheumatic arthritis, nephritises, and nyctalopia in China. In this study, fraction of the ethyl alcohol extract of E. densa (aerial part) by different polarity solvents indicated that the ethyl acetate soluble fraction exhibited a potent 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity with the IC50 value of 148.2 mu g/mL. Under the target guidance of DPPH experiment, isoquercitrin, trachelogenin, ethyl caffeate, and arctigenin were separated with purities 95.98%, 92.98%, 96.07%, and 88.83%, respectively, by a dual-mode high-speed counter-current chromatography (HSCCC) method using n-hexane-ethyl acetate-methanol-water (4.5:5:3:4, v/v/v/v) as the solvent system. In order to evaluate the scientific basis, antioxidant activity of four isolated compounds was assessed by the radical scavenging effect on DPPH radical; isoquercitrin and ethyl caffeate showed stronger antioxidant activities with IC50 values of 9.4 mu g/mL and 9.2 mu g/mL, respectively, while trachelogenin and arctigenin showed weak antioxidant activities with IC50 values of >500 mu g/mL and 72.8 mu g/mL, respectively. Results of the present study indicated that the combinative method using DPPH antioxidant assay and dual-mode HSCCC could be widely applied for rapid screening and isolating of antioxidants from complex traditional Chinese medicine extract.
关键词	Elsholtzia densa Benth. var. densa antioxidant target-guided dual-mode HSCCC
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59979
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhou, W. N.,Ouyang, J.,Wang, Z. H.,et al. Target-Guided Isolation and Purification of Antioxidants from Elsholtzia densa Benth. var. densa by DPPH Antioxidant Assay and Dual-Mode HSCCC[J]. ACTA CHROMATOGRAPHICA,2018,30(3).
APA	Zhou, W. N..,Ouyang, J..,Wang, Z. H..,Wang, X. Y..,Suo, Y. R..,...&Wang, H. L..(2018).Target-Guided Isolation and Purification of Antioxidants from Elsholtzia densa Benth. var. densa by DPPH Antioxidant Assay and Dual-Mode HSCCC.ACTA CHROMATOGRAPHICA,30(3).
MLA	Zhou, W. N.,et al."Target-Guided Isolation and Purification of Antioxidants from Elsholtzia densa Benth. var. densa by DPPH Antioxidant Assay and Dual-Mode HSCCC".ACTA CHROMATOGRAPHICA 30.3(2018).