beta-Elemene reduces the progression of atherosclerosis in rabbits

NWIPB OpenIR

	beta-Elemene reduces the progression of atherosclerosis in rabbits
	Zhong Ying 1; Liu Jun 1; Huo Wei-Min 3; Duan Wen-Li 1; Wang Xue 1; Shang Jing 1,2
	2015-06-01
发表期刊	CHINESE JOURNAL OF NATURAL MEDICINES
卷号	13 期号:6 页码:415-420
文章类型	Article
摘要	The present study aimed at investigating the possible effects of beta-elemene on the progression of atherosclerosis in a rabbit model. The rabbit atherosclerosis model was established. by the combination of balloon angioplasty-induced endothelial injury and an atherogenic diet fed to the rabbits. New Zealand White rabbits were randomly divided into four groups (8/group): the normal control group (fed with normal chow diet), and three experimental groups, placebo group, atorvastatin group, and beta-elemene group (received the atherogenic diet). After two weeks on the diet, the three experimental groups underwent balloon injury at right common carotid artery and were treated with drugs or placebo for five weeks. Serum lipids were measured. Carotid artery lesions were isolated for histological and immunohistochemical analysis. In vitro, RAW264.7 macrophages were pretreated with beta-elemene and ox-LDL for 24 h and the viability of macrophages was assayed using the MTT method. TNF-alpha and IL-6 were also determined. Compared with the control group, the thickness of the atherosclerosis lesion in the placebo group was significantly increased; The thickness the drug treatment groups were significantly decreased, compared with that of the placebo group. The infiltration of macrophage was markedly reduced in the beta-elemene group compared with that of the placebo group. beta-Elemene treatment also reduced the levels of TC, TG, and LDL-C, compared with the placebo group. beta-elemene decreased the TNF-alpha and IL-6 levels in vitro. In conclusion, our results demonstrated that beta-elemene retarded the progression of atherosclerosis in vivo and in vitro, which may be related to the capacity of beta-elemene to reduce the infiltration of macrophages and suppress inflammatory factors.; The present study aimed at investigating the possible effects of beta-elemene on the progression of atherosclerosis in a rabbit model. The rabbit atherosclerosis model was established. by the combination of balloon angioplasty-induced endothelial injury and an atherogenic diet fed to the rabbits. New Zealand White rabbits were randomly divided into four groups (8/group): the normal control group (fed with normal chow diet), and three experimental groups, placebo group, atorvastatin group, and beta-elemene group (received the atherogenic diet). After two weeks on the diet, the three experimental groups underwent balloon injury at right common carotid artery and were treated with drugs or placebo for five weeks. Serum lipids were measured. Carotid artery lesions were isolated for histological and immunohistochemical analysis. In vitro, RAW264.7 macrophages were pretreated with beta-elemene and ox-LDL for 24 h and the viability of macrophages was assayed using the MTT method. TNF-alpha and IL-6 were also determined. Compared with the control group, the thickness of the atherosclerosis lesion in the placebo group was significantly increased; The thickness the drug treatment groups were significantly decreased, compared with that of the placebo group. The infiltration of macrophage was markedly reduced in the beta-elemene group compared with that of the placebo group. beta-Elemene treatment also reduced the levels of TC, TG, and LDL-C, compared with the placebo group. beta-elemene decreased the TNF-alpha and IL-6 levels in vitro. In conclusion, our results demonstrated that beta-elemene retarded the progression of atherosclerosis in vivo and in vitro, which may be related to the capacity of beta-elemene to reduce the infiltration of macrophages and suppress inflammatory factors.
关键词	Beta-elemene Atherosclerosis Inflammation Macrophage Tnf-alpha
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	IN-VITRO ; CELLS ; INHIBITION ; EXPRESSION ; CARCINOMA ; MACROPHAGES ; ACTIVATION ; APOPTOSIS ; ALPHA ; VEGF
收录类别	SCI
语种	英语
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS类目	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000359754200003
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5543
专题	中国科学院西北高原生物研究所
作者单位	1.China Pharmaceut Univ, State Key Lab Nat Med, New Drug Screening Ctr, Nanjing 210009, Jiangsu, Peoples R China 2.China Pharmaceut Univ, Jiangsu Key Lab TCM Evaluat & Translat Res, Nanjing 210009, Jiangsu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Zhong Ying,Liu Jun,Huo Wei-Min,et al. beta-Elemene reduces the progression of atherosclerosis in rabbits[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2015,13(6):415-420.
APA	Zhong Ying,Liu Jun,Huo Wei-Min,Duan Wen-Li,Wang Xue,&Shang Jing.(2015).beta-Elemene reduces the progression of atherosclerosis in rabbits.CHINESE JOURNAL OF NATURAL MEDICINES,13(6),415-420.
MLA	Zhong Ying,et al."beta-Elemene reduces the progression of atherosclerosis in rabbits".CHINESE JOURNAL OF NATURAL MEDICINES 13.6(2015):415-420.