Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat

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	Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat
	Zhao, D.1,2,3; Derkx, A. P.2; Liu, D. -C.1; Buchner, P.2; Hawkesford, M. J.2
	2015-07-01
发表期刊	PLANT BIOLOGY
卷号	17 期号:4 页码:904-913
文章类型	Article
摘要	Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an AvalonxCadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.; Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an AvalonxCadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.
关键词	Grain Protein Deviation Nac Gene Family Nitrogen Remobilisation Rubisco Triticum Aestivum Yield
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	TRITICUM-AESTIVUM L. ; STAY-GREEN PHENOTYPE ; WINTER-WHEAT ; FACTOR FAMILY ; DURUM-WHEAT ; GENE GPC-B1 ; ARABIDOPSIS ; IDENTIFICATION ; REMOBILIZATION ; PROTEIN
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000356881400014
引用统计	被引频次：97[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5541
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China 2.Rothamsted Res, Plant Biol & Crop Sci Dept, Harpenden AL5 2JQ, Herts, England 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, D.,Derkx, A. P.,Liu, D. -C.,et al. Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat[J]. PLANT BIOLOGY,2015,17(4):904-913.
APA	Zhao, D.,Derkx, A. P.,Liu, D. -C.,Buchner, P.,&Hawkesford, M. J..(2015).Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat.PLANT BIOLOGY,17(4),904-913.
MLA	Zhao, D.,et al."Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat".PLANT BIOLOGY 17.4(2015):904-913.

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