Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC | |
| Zhang, Lin1; Yue, Hui-Lan1,2; Zhao, Xiao-Hui1,2,3; Li, Jing1; Shao, Yun1 | |
| 2015-07-01 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 卷号 | 53期号:6页码:860-865 |
| 文章类型 | Article |
| 摘要 | Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiede-mannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the first time. In the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for HSCCC separation. A total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside C, 22.7 mg of forsythoside B and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. The HSCCC fractions were analyzed by HPLC, and the chemical structures were identified by H-1 NMR and C-13 NMR. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of PPGs from natural products.; Four phenylpropanoid glycosides (PPGs), such as echinacoside, wiede-mannioside C, forsythoside B and verbascoside, were isolated and purified from the Tibetan medicinal herb Pedicularis longiflora Rudolph. var. tubiformis (Klotz) Tsoong by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation for the first time. In the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for HSCCC separation. A total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside C, 22.7 mg of forsythoside B and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. The HSCCC fractions were analyzed by HPLC, and the chemical structures were identified by H-1 NMR and C-13 NMR. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of PPGs from natural products. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; STACHYTARPHETA-CAYENNENSIS ; PHENYLETHANOID GLYCOSIDES ; CISTANCHE-DESERTICOLA ; PREPARATIVE ISOLATION ; LAMIOPHLOMIS-ROTATA ; PURIFICATION ; ANTIOXIDANT ; ROTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000361837600004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5537 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,et al. Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(6):860-865. |
| APA | Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,Li, Jing,&Shao, Yun.(2015).Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(6),860-865. |
| MLA | Zhang, Lin,et al."Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.6(2015):860-865. |
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