Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation

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	Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation
	Yue, Hui-Lan ; Zhao, Xiao-Hui ; Wang, Qi-Lan ; Tao, Yan-Duo ; Zhao, XH (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
	2013-10-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ; Yue, HL; Zhao, XH; Wang, QL; Tao, YD.Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,936():57-62
摘要	Four iridoid glucosides, shanzhiside methyl ester, phloyoside II, chlorotuberside, and penstemonoside, were isolated and purified from an herbal medicinal plant for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system composed of ethyl acetate-n-butanol-water (5:14:12, v/v/v). A total of 37 mg of shanzhiside methyl ester, 29 mg of phloyoside 11, 27 mg of chlorotuberside, and 21 mg of penstemonoside with the purity of 99.2%, 98.5%, 97.3%, and 99.3%, respectively, were obtained in one-step separation within 4 h from 150 mg of crude extract. To the best of our knowledge, this is the first report of separation and purification of iridoid glucosides from natural sources by HSCCC. The chemical structures of all the four compounds were identified by ESI-MS, H-1 NMR, and C-13 NMR. (C) 2013 Elsevier B.V. All rights reserved.; Four iridoid glucosides, shanzhiside methyl ester, phloyoside II, chlorotuberside, and penstemonoside, were isolated and purified from an herbal medicinal plant for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system composed of ethyl acetate-n-butanol-water (5:14:12, v/v/v). A total of 37 mg of shanzhiside methyl ester, 29 mg of phloyoside 11, 27 mg of chlorotuberside, and 21 mg of penstemonoside with the purity of 99.2%, 98.5%, 97.3%, and 99.3%, respectively, were obtained in one-step separation within 4 h from 150 mg of crude extract. To the best of our knowledge, this is the first report of separation and purification of iridoid glucosides from natural sources by HSCCC. The chemical structures of all the four compounds were identified by ESI-MS, H-1 NMR, and C-13 NMR. (C) 2013 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/52332
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yue, Hui-Lan,Zhao, Xiao-Hui,Wang, Qi-Lan,et al. Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Yue, HL; Zhao, XH; Wang, QL; Tao, YD.Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,936():57-62,2013.
APA	Yue, Hui-Lan,Zhao, Xiao-Hui,Wang, Qi-Lan,Tao, Yan-Duo,&Zhao, XH .(2013).Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.
MLA	Yue, Hui-Lan,et al."Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2013).