Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification | |
Wang, Wenli1; Li, Guoliang1; Ji, Zhongyin1,2; Hu, Na2; You, Jinmao1,2 | |
2014-08-01 | |
发表期刊 | FOOD ANALYTICAL METHODS |
ISSN | 1936-9751 |
卷号 | 7期号:7页码:1546-1556 |
文章类型 | Article |
摘要 | A stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability.; A stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability. |
关键词 | Aldehydes Hplc-fld-ms/ms Pre-column Derivatization Response Surface Methodology Foodstuffs |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; POLYCYCLIC AROMATIC-HYDROCARBONS ; CARBONYL-COMPOUNDS ; ALIPHATIC-ALDEHYDES ; GAS-CHROMATOGRAPHY ; SENSORY ANALYSIS ; DERIVATIZATION ; WINE ; 2,4-DINITROPHENYLHYDRAZINE |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Food Science & Technology |
WOS类目 | Food Science & Technology |
WOS记录号 | WOS:000338447800024 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/4207 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Wenli,Li, Guoliang,Ji, Zhongyin,et al. A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification[J]. FOOD ANALYTICAL METHODS,2014,7(7):1546-1556. |
APA | Wang, Wenli,Li, Guoliang,Ji, Zhongyin,Hu, Na,&You, Jinmao.(2014).A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification.FOOD ANALYTICAL METHODS,7(7),1546-1556. |
MLA | Wang, Wenli,et al."A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification".FOOD ANALYTICAL METHODS 7.7(2014):1546-1556. |
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