Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk | |
| Hu, Xi-Gui1,2; Wu, Bi-Hua1,2; Liu, Deng-Cai1,2,3; Wei, Yu-Ming1,2; Gao, Shi-Bin2,4; Zhen, You-Liang1,2; Wu, BH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China. | |
| 2013-02-15 | |
| 发表期刊 | JOURNAL OF PLANT PHYSIOLOGY
 |
| ISSN | 0176-1617 |
| 卷号 | 170期号:3页码:330-337 |
| 文章类型 | Article |
| 摘要 | NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n = 4x = 28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Korn. (AABB, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.; NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n = 4x = 28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Korn. (AABB, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved. |
| 关键词 | T. T.mopheevii Nam-g1 Gene Grain Protein Content |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.jplph.2012.10.009 |
| 关键词[WOS] | NAC TRANSCRIPTION FACTORS ; CONTENT LOCUS ; WHEAT ; ARABIDOPSIS ; FAMILY ; DOMAIN ; DOMESTICATION ; SUPERFAMILY ; SENESCENCE ; HORDEUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(30571139 ; Personnel Training Foundation ; Education Committee Accented Term in Sichuan Province ; 30671271) |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000315310000012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3960 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Wu, BH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China. |
| 作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China 2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Yaan, Peoples R China 3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 4.Sichuan Agr Univ, Maize Res Inst, Wenjiang 611130, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xi-Gui,Wu, Bi-Hua,Liu, Deng-Cai,et al. Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(3):330-337. |
| APA | Hu, Xi-Gui.,Wu, Bi-Hua.,Liu, Deng-Cai.,Wei, Yu-Ming.,Gao, Shi-Bin.,...&Wu, BH .(2013).Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk.JOURNAL OF PLANT PHYSIOLOGY,170(3),330-337. |
| MLA | Hu, Xi-Gui,et al."Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk".JOURNAL OF PLANT PHYSIOLOGY 170.3(2013):330-337. |
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