Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Analysis of estrogenic compounds in environmental and biological samples by liquid chromatography-tandem mass spectrometry with stable isotope-coded ionization-enhancing reagent | |
| Zhang, Shijuan1,3; You, Jinmao1,2; Ning, Shujing2; Song, Cuihua2; Suo, You-Rui1; You, JM (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China. | |
| 2013-03-08 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHY A
 |
| ISSN | 0021-9673 |
| 卷号 | 1280页码:84-91 |
| 文章类型 | Article |
| 摘要 | A sensitive and reliable stable isotope labeling technology was developed for the determination of estrogenic compounds in environmental and biological samples based on the derivatization of estrogenic compounds with 10-methyl-acridone-2-sulfonyl chloride (d(0)-MASC) and its deuterated counterpart d(3)-MASC. The labeling reaction of MASC with estrogenic compounds is simple and robust and can be carried out under mild conditions within 5 min. Internal standard-based quantification was achieved by this labeling strategy without the need of using expensive internal standard analogy to every analyte of interest. Meanwhile, the sensitivity obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was enhanced by 2-3 orders of magnitude compared to the underivatized counterparts. Application of the stable isotope labeling technology in relative and absolute quantification of estrogenic compounds in complicated samples indicated that the labeling strategy was effective in overcoming matrix effects. The proposed method was successfully applied to the analysis estrogenic compounds in different environmental and biological samples with high sensitivity and accuracy. (c) 2013 Elsevier B.V. All rights reserved.; A sensitive and reliable stable isotope labeling technology was developed for the determination of estrogenic compounds in environmental and biological samples based on the derivatization of estrogenic compounds with 10-methyl-acridone-2-sulfonyl chloride (d(0)-MASC) and its deuterated counterpart d(3)-MASC. The labeling reaction of MASC with estrogenic compounds is simple and robust and can be carried out under mild conditions within 5 min. Internal standard-based quantification was achieved by this labeling strategy without the need of using expensive internal standard analogy to every analyte of interest. Meanwhile, the sensitivity obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was enhanced by 2-3 orders of magnitude compared to the underivatized counterparts. Application of the stable isotope labeling technology in relative and absolute quantification of estrogenic compounds in complicated samples indicated that the labeling strategy was effective in overcoming matrix effects. The proposed method was successfully applied to the analysis estrogenic compounds in different environmental and biological samples with high sensitivity and accuracy. (c) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Estrogenic Compounds Stable Isotope Labeling Lc-ms/ms Matrix Effects |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.chroma.2013.01.045 |
| 关键词[WOS] | SOLID-PHASE EXTRACTION ; ENDOCRINE-DISRUPTING COMPOUNDS ; ELECTROSPRAY-IONIZATION ; GAS-CHROMATOGRAPHY ; LC-MS/MS ; RELATIVE QUANTIFICATION ; SEWAGE-SLUDGE ; ESI-MS ; DERIVATIZATION ; WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | 100 Talents Program of the Chinese Academy of Sciences(328) ; National Science Foundation of China(21275089) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000315758700009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3954 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | You, JM (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shijuan,You, Jinmao,Ning, Shujing,et al. Analysis of estrogenic compounds in environmental and biological samples by liquid chromatography-tandem mass spectrometry with stable isotope-coded ionization-enhancing reagent[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1280:84-91. |
| APA | Zhang, Shijuan,You, Jinmao,Ning, Shujing,Song, Cuihua,Suo, You-Rui,&You, JM .(2013).Analysis of estrogenic compounds in environmental and biological samples by liquid chromatography-tandem mass spectrometry with stable isotope-coded ionization-enhancing reagent.JOURNAL OF CHROMATOGRAPHY A,1280,84-91. |
| MLA | Zhang, Shijuan,et al."Analysis of estrogenic compounds in environmental and biological samples by liquid chromatography-tandem mass spectrometry with stable isotope-coded ionization-enhancing reagent".JOURNAL OF CHROMATOGRAPHY A 1280(2013):84-91. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Analysis of estrogen（705KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论