Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC | |
| Zhao, Xiao-Hui1,2; Han, Fa1; Li, Yu-Lin1; Zhou, Guo-Ying1; Yue, Hui-Lan1; Yue, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. | |
| 2013-05-01 | |
| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| ISSN | 1082-6076 |
| 卷号 | 36期号:13页码:1751-1761 |
| 文章类型 | Article |
| 摘要 | An effective highspeed countercurrent chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by H-1 NMR and C-13 NMR.; An effective highspeed countercurrent chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by H-1 NMR and C-13 NMR. |
| 关键词 | Apigenin Chrysoeriol Flavonoid Hsccc Luteolin Pedicularis Longiflora Var Tubiformis (Klotzsch) p c Tsoong |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1080/10826076.2012.698677 |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; G(2)/M ARREST ; APIGENIN ; APOPTOSIS ; LEAVES ; CELLS ; EFFICIENT ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Science and Technology Support Program of China(2007BAC30B04) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000318779800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3946 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Yue, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,et al. SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2013,36(13):1751-1761. |
| APA | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,Zhou, Guo-Ying,Yue, Hui-Lan,&Yue, HL .(2013).SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,36(13),1751-1761. |
| MLA | Zhao, Xiao-Hui,et al."SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 36.13(2013):1751-1761. |
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