Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection | |
Li, Yanxin1; Zhang, Shijuan2,3; Song, Cuihua1; You, Jinmao1; You, JM (reprint author), Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Peoples R China. | |
2013-10-01 | |
发表期刊 | FOOD ANALYTICAL METHODS |
ISSN | 1936-9751 |
卷号 | 6期号:5页码:1284-1290 |
文章类型 | Article |
摘要 | A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods.; A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods. |
关键词 | Bisphenol a Alkylphenol Hplc Fluorescence |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s12161-012-9541-0 |
关键词[WOS] | TANDEM MASS-SPECTROMETRY ; ENDOCRINE-DISRUPTING COMPOUNDS ; SOLID-PHASE EXTRACTION ; WASTE-WATER ; CANNED VEGETABLES ; LABELING REAGENT ; MILK ; GAS ; MICROEXTRACTION ; DERIVATIZATION |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Chinese Academy of Sciences(328) ; National Science Foundation of China(21275089) |
WOS研究方向 | Food Science & Technology |
WOS类目 | Food Science & Technology |
WOS记录号 | WOS:000324271200006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/3922 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | You, JM (reprint author), Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Peoples R China. |
作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Plateau Inst Biol, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Yanxin,Zhang, Shijuan,Song, Cuihua,et al. Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection[J]. FOOD ANALYTICAL METHODS,2013,6(5):1284-1290. |
APA | Li, Yanxin,Zhang, Shijuan,Song, Cuihua,You, Jinmao,&You, JM .(2013).Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection.FOOD ANALYTICAL METHODS,6(5),1284-1290. |
MLA | Li, Yanxin,et al."Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection".FOOD ANALYTICAL METHODS 6.5(2013):1284-1290. |
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