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The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring | |
Yuan, Zhongwei ; Liu, Miao ; Ouyang, Yuyuan ; Zeng, Xiaoxue ; Hao, Ming ; Zhang, Lianquan ; Ning, Shunzong ; Yan, Zehong ; Liu, Dengcai | |
2014-10-01 | |
发表期刊 | THEORETICAL AND APPLIED GENETICS ; Yuan, ZW; Liu, M; Ouyang, YY; Zeng, XX; Hao, M; Zhang, LQ; Ning, SZ; Yan, ZH; Liu, DC.The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring,THEORETICAL AND APPLIED GENETICS,2014,127(10):2173 |
摘要 | This study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. Wide hybridization can produce de novo DNA variation that may cause novel phenotypes. However, there is still a lack of specific links between changed genes and novel phenotypes in wide hybrids. The well-studied high-molecular-weight glutenin subunit (HMW-GS) genes in tribe Triticeae provide a useful model for addressing this issue. In this study, we investigated the feasibility of a wheat-rye hybridization method for inducing de novo phenotypes using the Glu-1Dx2.2 subunit as an example. We developed three hexaploid wheat lines with normal fertility and a Glu-1Dx2.2 variant, named Glu-1Dx2.2 (v) , derived from three F-1 hybrids. The wild-type Glu-1Dx2.2 has two direct repeats of 295 bp length separated by an intervening 101 bp in its central repetitive region. In the mutant Glu-1Dx2.2 (v) , one copy of the repeats and the intervening sequence were deleted, probably through homology-dependent illegitimate recombination (IR). This study provides a direct link between a de novo allele and novel phenotype. Our results indicate that the wheat-rye method may be a useful tool to induce de novo genetic variations that broaden the genetic diversity for wheat improvement.; This study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/37341 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Yuan, Zhongwei,Liu, Miao,Ouyang, Yuyuan,et al. The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring[J]. THEORETICAL AND APPLIED GENETICS, Yuan, ZW; Liu, M; Ouyang, YY; Zeng, XX; Hao, M; Zhang, LQ; Ning, SZ; Yan, ZH; Liu, DC.The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring,THEORETICAL AND APPLIED GENETICS,2014,127(10):2173,2014. |
APA | Yuan, Zhongwei.,Liu, Miao.,Ouyang, Yuyuan.,Zeng, Xiaoxue.,Hao, Ming.,...&Liu, Dengcai.(2014).The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring.THEORETICAL AND APPLIED GENETICS. |
MLA | Yuan, Zhongwei,et al."The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring".THEORETICAL AND APPLIED GENETICS (2014). |
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