Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Selectively screen the antibacterial peptide from the hydrolysates of highland barley | |
Pei, Jinjin ; Feng, Zhenzhen ; Ren, Ting ; Jin, Wengang ; Li, Xinsheng ; Chen, Dejing ; Tao, Yanduo ; Dang, Jun | |
2018 | |
发表期刊 | ENGINEERING IN LIFE SCIENCES |
摘要 | Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane. |
出版者 | WILEY |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/23745 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018. |
APA | Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES. |
MLA | Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES (2018). |
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