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Determination of Fatty Acids (C1-C10) from Bryophytes and Pteridophytes | |
Xin-Wei Bai; Cui-Hua Song; Jin-Mao You; Zhi-Wei Sun; Yan-Yan Fu; Guo-Liang Li | |
2010 | |
发表期刊 | Chromatographia |
ISSN | 0009-5893 |
卷号 | 71期号:11页码:1125-1129 |
摘要 | A simple and mild method for the determination of fatty acids (C1 – C10) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophoresis has been developed. The detection was performed with a diode array detector at 254 nm. A 58.5 cm × 50 μm i.d. (50 cm effective length) untreated fused-silica capillary was used. To optimize the separation conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The optimal separation conditions were as follows: 30 mmol L?1 borate buffer (pH 9.5), 15 mmol L?1 β-CD, temperature at 20 °C, pressure 50 mbar and injection time 8 s. Under the established conditions, 10 fatty acid derivatives could be well-separated within 17 min. The linearity was in the range of 0.07–5.0 μmol L?1. Detection limits (at a signal-to-noise ratio of 3) were in the range of 0.027–0.042 μmol L?1. The fatty acids from the extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results. |
收录类别 | SCI |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/2006 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Xin-Wei Bai,Cui-Hua Song,Jin-Mao You,et al. Determination of Fatty Acids (C1-C10) from Bryophytes and Pteridophytes[J]. Chromatographia,2010,71(11):1125-1129. |
APA | Xin-Wei Bai,Cui-Hua Song,Jin-Mao You,Zhi-Wei Sun,Yan-Yan Fu,&Guo-Liang Li.(2010).Determination of Fatty Acids (C1-C10) from Bryophytes and Pteridophytes.Chromatographia,71(11),1125-1129. |
MLA | Xin-Wei Bai,et al."Determination of Fatty Acids (C1-C10) from Bryophytes and Pteridophytes".Chromatographia 71.11(2010):1125-1129. |
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