Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC | |
Chen, Chen ; Zhao, Xiao-Hui ; Yue, Hui-Lan ; Li, Yu-Lin ; Chen, Tao | |
2014-05-01 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE ; Chen, C; Zhao, XH; Yue, HL; Li, YL; Chen, T.Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC,JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):395 |
摘要 | An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.; An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/17001 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,et al. Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, Chen, C; Zhao, XH; Yue, HL; Li, YL; Chen, T.Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC,JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):395,2014. |
APA | Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,Li, Yu-Lin,&Chen, Tao.(2014).Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE. |
MLA | Chen, Chen,et al."Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2014). |
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