Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes | |
Bai, XW; Song, CH; You, JM; Sun, ZW; Fu, YY; Li, GL | |
2010 | |
发表期刊 | CHROMATOGRAPHIA |
ISSN | 0009-5893 |
卷号 | 71期号:11-12页码:1125-1129 |
摘要 | A simple and mild method for the determination of fatty acids (C(1) - C(10)) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophoresis has been developed. The detection was performed with a diode array detector at 254 nm. A 58.5 cm x 50 mu m i.d. (50 cm effective length) untreated fused-silica capillary was used. To optimize the separation conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The optimal separation conditions were as follows: 30 mmol L(-1) borate buffer (pH 9.5), 15 mmol L(-1) beta-CD, temperature at 20 A degrees C, pressure 50 mbar and injection time 8 s. Under the established conditions, 10 fatty acid derivatives could be well-separated within 17 min. The linearity was in the range of 0.07-5.0 mu mol L(-1). Detection limits (at a signal-to-noise ratio of 3) were in the range of 0.027-0.042 mu mol L(-1). The fatty acids from the extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results. |
关键词 | Capillary Zone Electrophoresis Pre-column Derivation Fatty Acids Funaria Hedw. And Selaginella |
收录类别 | sci |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1682 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Bai, XW,Song, CH,You, JM,et al. Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes[J]. CHROMATOGRAPHIA,2010,71(11-12):1125-1129. |
APA | Bai, XW,Song, CH,You, JM,Sun, ZW,Fu, YY,&Li, GL.(2010).Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes.CHROMATOGRAPHIA,71(11-12),1125-1129. |
MLA | Bai, XW,et al."Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes".CHROMATOGRAPHIA 71.11-12(2010):1125-1129. |
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