Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization | |
| Dou, Quan-Wen1; Zhang, Tong-Lin2; Tsujimoto, Hisashi3 | |
| 2011-07-01 | |
| 发表期刊 | JOURNAL OF SYSTEMATICS AND EVOLUTION
 |
| ISSN | 1674-4918 |
| 卷号 | 49期号:4页码:347-352 |
| 文章类型 | Article |
| 摘要 | Genome constitution and genetic relationships between six Elymus species were assessed by physical mapping of different repetitive sequences using a technique of sequential fluorescence in situ hybridization and genomic in situ hybridization. The six Elymus species are all naturally growing species in northwest China, namely, E. sibiricus, E. nutans, E. barystachyus, E. xiningensis, E. excelsus, and E. dahuricus. An StStHH genome constitution was revealed for E. sibiricus and StStHHYY for the remainder species. Each chromosome could be clearly characterized by physical mapping with 18S-26S rDNA, 5S rDNA, Afa-family, and AAG repeats, and be allocated to a certain genome by genomic in situ hybridization. Two 5S rDNA sites, each in the H and St genomes, and three 18S-26S rDNA sites, two in the St genome and one in the Y genome, were uncovered in most of the species. The strong Afa-family hybridization signals discriminated the H genome from the St and Y genomes. The H and Y genome carried more AAG repeats than St. A common non-Robertsonian reciprocal translocation between the H and Y genomes was revealed in E. barystachyus, E. xiningensis, E. excelsus and E. dahuricus. Comparison of molecular karyotypes strongly suggests that they can be classified into three groups, namely, E. sibiricus, E. nutans, and others.; Genome constitution and genetic relationships between six Elymus species were assessed by physical mapping of different repetitive sequences using a technique of sequential fluorescence in situ hybridization and genomic in situ hybridization. The six Elymus species are all naturally growing species in northwest China, namely, E. sibiricus, E. nutans, E. barystachyus, E. xiningensis, E. excelsus, and E. dahuricus. An StStHH genome constitution was revealed for E. sibiricus and StStHHYY for the remainder species. Each chromosome could be clearly characterized by physical mapping with 18S-26S rDNA, 5S rDNA, Afa-family, and AAG repeats, and be allocated to a certain genome by genomic in situ hybridization. Two 5S rDNA sites, each in the H and St genomes, and three 18S-26S rDNA sites, two in the St genome and one in the Y genome, were uncovered in most of the species. The strong Afa-family hybridization signals discriminated the H genome from the St and Y genomes. The H and Y genome carried more AAG repeats than St. A common non-Robertsonian reciprocal translocation between the H and Y genomes was revealed in E. barystachyus, E. xiningensis, E. excelsus and E. dahuricus. Comparison of molecular karyotypes strongly suggests that they can be classified into three groups, namely, E. sibiricus, E. nutans, and others. |
| 关键词 | Elymus Fluorescence In Situ Hybridization Repetitive Sequences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | RIBOSOMAL-RNA GENES ; AFA-FAMILY ; BARLEY CHROMOSOMES ; TRITICEAE ; POACEAE ; WHEAT ; IDENTIFICATION ; NUTANS ; LOCI ; FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000293273200005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1532 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810008, Peoples R China 2.Jiangxi Inst Educ, Nanchang 330029, Peoples R China 3.Tottori Univ, Fac Agr, Tottori 6808550, Japan |
| 推荐引用方式 GB/T 7714 | Dou, Quan-Wen,Zhang, Tong-Lin,Tsujimoto, Hisashi. Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(4):347-352. |
| APA | Dou, Quan-Wen,Zhang, Tong-Lin,&Tsujimoto, Hisashi.(2011).Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization.JOURNAL OF SYSTEMATICS AND EVOLUTION,49(4),347-352. |
| MLA | Dou, Quan-Wen,et al."Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.4(2011):347-352. |
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